s. cerevisiae bcrc 21685  (BioResource International Inc)

Journal: Microorganisms

Article Title: Construction of Recombinant Saccharomyces cerevisiae with Ethanol and Aldehydes Tolerance via Overexpression of Aldehyde Reductase

doi: 10.3390/microorganisms10050850

Figure Lengend Snippet: ( A ) PCR confirmation of the recombinant expression vector pGAPZC-ari1 in SC and SCA. ( B ) Overexpression of ARI in SC and SCA. SC ( S. cerevisiae ) and SCA ( S. cerevisiae with ari1 gene overexpression).

Article Snippet: S. cerevisiae (BCRC 21685) was purchased from the Bioresource Collection and Research Center, Food Industry Research and Development Institution, Shinchu, Taiwan.

Techniques: Recombinant, Expressing, Plasmid Preparation, Over Expression

Journal: Microorganisms

Article Title: Construction of Recombinant Saccharomyces cerevisiae with Ethanol and Aldehydes Tolerance via Overexpression of Aldehyde Reductase

doi: 10.3390/microorganisms10050850

Figure Lengend Snippet: Furfural tolerance analysis of Saccharomyces cerevisiae. Solid lines represent engineered strains; dashed lines represent wild strains.

Article Snippet: S. cerevisiae (BCRC 21685) was purchased from the Bioresource Collection and Research Center, Food Industry Research and Development Institution, Shinchu, Taiwan.

Techniques:

Journal: Microorganisms

Article Title: Construction of Recombinant Saccharomyces cerevisiae with Ethanol and Aldehydes Tolerance via Overexpression of Aldehyde Reductase

doi: 10.3390/microorganisms10050850

Figure Lengend Snippet: HMF tolerance analysis of Saccharomyces cerevisiae . Solid lines represent engineered strains; dashed lines represent wild strains.

Article Snippet: S. cerevisiae (BCRC 21685) was purchased from the Bioresource Collection and Research Center, Food Industry Research and Development Institution, Shinchu, Taiwan.

Techniques:

Journal: Microorganisms

Article Title: Construction of Recombinant Saccharomyces cerevisiae with Ethanol and Aldehydes Tolerance via Overexpression of Aldehyde Reductase

doi: 10.3390/microorganisms10050850

Figure Lengend Snippet: Ari gene expression (bar chart) and cell growth (graph) of SCA (filled symbol) and SC (open symbol) on the YPD medium ( A ), YPD + 20 mM furfural ( B ) and YPD + 60 mM HMF ( C ). SC ( S. cerevisiae ) and SCA ( S. cerevisiae with ari1 gene overexpression).

Article Snippet: S. cerevisiae (BCRC 21685) was purchased from the Bioresource Collection and Research Center, Food Industry Research and Development Institution, Shinchu, Taiwan.

Techniques: Gene Expression, Over Expression

Journal: Microorganisms

Article Title: Construction of Recombinant Saccharomyces cerevisiae with Ethanol and Aldehydes Tolerance via Overexpression of Aldehyde Reductase

doi: 10.3390/microorganisms10050850

Figure Lengend Snippet: Ari gene expression (bar chart) and aldehyde degradation (line chart) of SCA (filled symbol) and SC (open symbol) in the presence of 60 mM HMF (Blue color) or 20 mM Furfural (Red color) on the YPD medium. SC ( S. cerevisiae ) and SCA ( S. cerevisiae with ari1 gene overexpression).

Article Snippet: S. cerevisiae (BCRC 21685) was purchased from the Bioresource Collection and Research Center, Food Industry Research and Development Institution, Shinchu, Taiwan.

Techniques: Gene Expression, Over Expression

Journal: Microorganisms

Article Title: Construction of Recombinant Saccharomyces cerevisiae with Ethanol and Aldehydes Tolerance via Overexpression of Aldehyde Reductase

doi: 10.3390/microorganisms10050850

Figure Lengend Snippet: Ethanol conversion rate and production using SCA (filled symbol) and SC (open symbol) in the presence of 10% glucose on the YPD medium. SC ( S. cerevisiae ) and SCA ( S. cerevisiae with ari1 gene overexpression).

Article Snippet: S. cerevisiae (BCRC 21685) was purchased from the Bioresource Collection and Research Center, Food Industry Research and Development Institution, Shinchu, Taiwan.

Techniques: Over Expression

Journal: Bioengineered

Article Title: Engineering Saccharomyces cerevisiae for improvement in ethanol tolerance by accumulation of trehalose

doi: 10.1080/21655979.2016.1207019

Figure Lengend Snippet: Strains used in this study.

Article Snippet: S. cerevisiae (BCRC 21685) was purchased from Bioresource Collection and Research Center, Food Industry Research and Development Institution, Hsinchu, Taiwan.

Techniques: Over Expression, Plasmid Preparation

Journal: Bioengineered

Article Title: Engineering Saccharomyces cerevisiae for improvement in ethanol tolerance by accumulation of trehalose

doi: 10.1080/21655979.2016.1207019

Figure Lengend Snippet: Construction of deletion cassette with long flanking homology regions by overlapping PCR. In the first round of PCR, the upstream sequence of the nth1 gene, the kanr marker gene and the downstream sequence of the nth1 gene were amplified in 3 different PCR reaction tubes. S. cerevisiae genomic DNA was used as template for amplification of 0.26 kb 5′ UP nth1 region and 0.4 kb 3′ DOWN nth1 region by using primers L1, L2, and L3, L4, respectively. The pFA6a-kanMX6 vector was used as a template for amplification of kanMX region by using primers kanMX-F and kanMX-R. In the second round of PCR, the 5′ UP nth1 region and kanMX region were fused together by using primers L1 and kanMX-R. In the third round of PCR, the 3′ DOWN nth1 region and second round PCR product (5′ UP nth1 region-kanMX region) were fused together by using primers L1 and L4. The third round PCR product (5′ UP nth1 region- kanMX region - 3′ DOWN nth1 region) was cloned into pGEMT vector for construction of the disruption cassette. After transformation of the disruption cassette into S. cerevisiae, homologous recombination takes place with the deletion of target gene.

Article Snippet: S. cerevisiae (BCRC 21685) was purchased from Bioresource Collection and Research Center, Food Industry Research and Development Institution, Hsinchu, Taiwan.

Techniques: Sequencing, Marker, Amplification, Plasmid Preparation, Clone Assay, Transformation Assay, Homologous Recombination